Professor
Email:youhuijuan@@hust.edu.cn
Academic Areas: Single-molecule biophysics, Molecular Pharmacology, Nucleic acids, G-quadruplexes
Research Interests: Drugs targeting G-quadruplexes, including anti-cancer drugs, Development of drug screening methods based on single-molecule techniques.
Academic Degrees
PhD in Biotechnology, Graduate School of Engineering, 2012, Osaka University, Osaka, Japan;
Master in Biotechnology, Graduate School of Engineering, 2008, Osaka University, Osaka, Japan;
Bachelor in Biological Science, Peking University, 2006, Beijing, China.
Professional Experience
Professor (2017-present); School of Pharmaceutical Science, Tongji Medical College, HUST
Research fellow (2013-2017); Mechanobiology Institute, National university of Singapore, Singapore
Project Academic Support Specialist (2011-2013); Department of applied chemistry, School of Engineering, The University of Tokyo, Japan
Selected Publications
1. Zhang, Y#., Cheng, Y#., Chen, J., Zheng K*.,You, H*. Mechanical diversity and folding intermediates of parallel-stranded G-quadruplexes with a bulge. Nucleic Acids Research, 2021, https://doi.org/10.1093/nar/gkab531
2. Cheng, Y., Zhang, Y., Gong, Z., Zhang, X., Li, Y., Shi, X., Yufeng Pei.,You, H*. High Mechanical Stability and Slow Unfolding Rates Are Prevalent in Parallel-Stranded DNA G-Quadruplexes. The Journal of Physical Chemistry Letters, 2020, 11(19): 7966-7971.
3. Pei, Y., Liu, Y., Xie, C., Zhang, X.,You, H*. Detecting the Formation Kinetics of Doxorubicin-DNA Interstrand Cross-link at the Single-Molecule Level and Clinically Relevant Concentrations of Doxorubicin. Analytical Chemistry. 2020, 92 (6), 4504-4511.
4. Li, X., Pei, Y., Zhang, Y., Liu, Y., Fu, W., Li, J., You,H*., Huang, J*. (2020). Single-Molecule Mechanical Unfolding of AT-Rich Chromosomal Fragile Site DNA Hairpins: Resolving the Thermodynamic and Kinetic Effects of a Single GT Mismatch. The Journal of Physical Chemistry B, 124(42), 9365-9370.
5. Cheng, Y., Tang, Q., Li, Y., Zhang, Y., Zhao, C., Yan, J.,You, H*. Folding/unfolding kinetics of G-quadruplexes upstream of the P1 promoter of the human BCL-2 oncogene. Journal of Biological Chemistry. 2019 294 (15), 5890-5895.
6. Tong, Q#.,You, H#., Chen, X., Wang, K., Sun, W., Pei, Y., Zhao, X., Yuan, M., Zhu, H., Luo, Z*., Zhang, Y*. ZYH005, a novel DNA intercalator, overcomes all-trans retinoic acid resistance in acute promyelocytic leukemia. Nucleic Acids Research. 2018, 46(7): 3284-3297.
7.You, H#., Guo, S#., Le, S., Tang, Q., Yao, M., Zhao, X., Yan, J*.. Two-State Folding Energy Determination Based on Transition Points in Non-Equilibrium Single-Molecule Experiments. The journal of physical chemistry letters. 2018, 9 (4), 811–816.
8.You, H#., Lattmann, S#., Rhodes, D*., Yan, J*. RHAU helicase stabilizes G4 in its nucleotide-free state and destabilizes G4 upon ATP hydrolysis. Nucleic Acids Research, 2017, 45(1): 206-214.
9.You, H.,Wu, J., Shao, F., Yan, J*.. Stability and kinetics of c-MYC promoter G-quadruplexes studied by single-molecule manipulation. Journal of the American Chemical Society, 2015, 137 (7), 2424-2427.
10. You, H., Zeng, X., Xu, Y., Lim C.J, Efremov, A., Phan A.T, Yan J*.. Dynamics and stability of polymorphic human telomeric G-quadruplex under tension. Nucleic Acids Research. 2014, 42 (13), 8789-8795.
11.You, H., Iino, R., Watanabe, R., Noji, H*.. Winding single-molecule double-stranded DNA on a nanometer-sized reel. Nucleic Acids Research. 2012 40 (19): e151.
Reviews
1. Cheng, Y., Zhang, Y., & You, H. (2021). Characterization of G-Quadruplexes Folding/Unfolding Dynamics and Interactions with Proteins from Single-Molecule Force Spectroscopy. Biomolecules, 11(11), 1579.
2. You, H., Zhou, Y., & Yan, J. (2021). Using Magnetic Tweezers to Unravel the Mechanism of the G-quadruplex Binding and Unwinding Activities of DHX36 Helicase. In RNA Remodeling Proteins (pp. 175-191). Humana, New York, NY.
Courses Taught
Pharmacology
